| 1 | | | | | | 25 | Schneider Skylar Miami Blazers | Miami Blazers | | 1:16:221:16:22 |
| 2 | | | | | | 20 | MacPherson Makayla DNA Pro Cycling | DNA Pro Cycling | | ,,0:00 |
| 3 | | | | | | 31 | Mejías Marlies Virginia's Blue Ridge - TWENTY24 | Virginia's Blue Ridge - TWENTY24 | | ,,0:00 |
| 4 | | | | | | 26 | Easler Maeghan DNA Pro Cycling | DNA Pro Cycling | | ,,0:00 |
| 5 | | | | | | 19 | Patrick Chloe Cynisca Cycling | Cynisca Cycling | | ,,0:00 |
| 6 | | | | | | 20 | Hickey Cassidy Denver Disruptors | Denver Disruptors | | ,,0:00 |
| 7 | | | | | | 31 | Halamek Stephanie | | | ,,0:00 |
| 8 | | | | | | 32 | Arreola Sofía Virginia's Blue Ridge - TWENTY24 | Virginia's Blue Ridge - TWENTY24 | | ,,0:00 |
| 9 | | | | | | 33 | Schneider Samantha Miami Blazers | Miami Blazers | | ,,0:00 |
| 10 | | | | | | 26 | Villamizar Karen Lorena Boneshaker Project presented by ORANGE SEAL | Boneshaker Project presented by ORANGE SEAL | | ,,0:00 |
| 11 | | | | | | | Obrand Alexandrine Terun Elite | Terun Elite | | ,,0:00 |
| 12 | | | | | | 20 | Roldan Mara Cynisca Cycling | Cynisca Cycling | | ,,0:00 |
| 13 | | | | | | 33 | Dixon Elizabeth Fount Cycling Guild | Fount Cycling Guild | | ,,0:00 |
| 14 | | | | | | 22 | Clouse Katie | | | ,,0:00 |
| 15 | | | | | | 37 | Buttine Andrea Fount Cycling Guild | Fount Cycling Guild | | ,,0:00 |
| 16 | | | | | | 20 | Sarkisov Katherine Cynisca Cycling | Cynisca Cycling | | ,,0:00 |
| 17 | | | | | | 26 | Lejeune Cecile CCB p/b Levine Law Group | CCB p/b Levine Law Group | | ,,0:00 |
| 18 | | | | | | 21 | Barraclough Ngaire Boneshaker Project presented by ORANGE SEAL | Boneshaker Project presented by ORANGE SEAL | | ,,0:00 |
| 19 | | | | | | 30 | Bolard Galen Competitive Edge Racing | Competitive Edge Racing | | ,,0:00 |
| 20 | | | | | | 19 | Francis Jenaya Grouwels-Watersley R&D Road Team | Grouwels-Watersley R&D Road Team | | ,,0:00 |
| 21 | | | | | | 41 | Coney Helena | | | ,,0:00 |
| 22 | | | | | | 28 | Shafi Alia Fount Cycling Guild | Fount Cycling Guild | | ,,0:00 |
| 23 | | | | | | 38 | Karagianis Daphne Denver Disruptors | Denver Disruptors | | ,,0:00 |
| 24 | | | | | | 36 | Kuyk-White Taylor | | | ,,0:00 |
| 25 | | | | | | 22 | Seiler Kate CCB p/b Levine Law Group | CCB p/b Levine Law Group | | ,,0:00 |
| 26 | | | | | | 31 | Breck Holly DNA Pro Cycling | DNA Pro Cycling | | ,,0:00 |
| 27 | | | | | | 29 | Minagawa Minori Fount Cycling Guild | Fount Cycling Guild | | ,,0:00 |
| 28 | | | | | | 30 | Powless Shayna DNA Pro Cycling | DNA Pro Cycling | | ,,0:00 |
| 29 | | | | | | 24 | Walcroft Katelyn Competitive Edge Racing | Competitive Edge Racing | | ,,0:00 |
| 30 | | | | | | 27 | Rusch Katherine CCB p/b Levine Law Group | CCB p/b Levine Law Group | | ,,0:00 |
| 31 | | | | | | 24 | Windsor Claire Cynisca Cycling | Cynisca Cycling | | ,,0:00 |
| 32 | | | | | | 24 | Ramirez Andrea Boneshaker Project presented by ORANGE SEAL | Boneshaker Project presented by ORANGE SEAL | | ,,0:00 |
| 33 | | | | | | 32 | Dorovskikh Anna Orion Racing | Orion Racing | | ,,0:00 |
| 34 | | | | | | 27 | Poidevin Sara DNA Pro Cycling | DNA Pro Cycling | | ,,0:00 |
| 35 | | | | | | 30 | O'Boyle Megan | | | ,,0:00 |
| 36 | | | | | | | Lang Rebecca Terun Elite | Terun Elite | | ,,0:00 |
| 37 | | | | | | 33 | Bertold Isabella | | | ,,0:00 |
| 38 | | | | | | 30 | Fraser Chloe | | | ,,0:00 |
| 39 | | | | | | 23 | Boglio Cassia | | | ,,0:00 |
| 40 | | | | | | 28 | Howden Florence United Cycling | United Cycling | | ,,0:00 |
| 41 | | | | | | 29 | Scott Dana | | | ,,0:00 |
| 42 | | | | | | 37 | Peñuela Diana DNA Pro Cycling | DNA Pro Cycling | | ,,0:00 |
| 43 | | | | | | 20 | Aseltine Mia Competitive Edge Racing | Competitive Edge Racing | | ,,0:00 |
| 44 | | | | | | 28 | Rollins Melisa Virginia's Blue Ridge - TWENTY24 | Virginia's Blue Ridge - TWENTY24 | | ,,0:00 |
| 45 | | | | | | 23 | Gontova Nadia DNA Pro Cycling | DNA Pro Cycling | | ,,0:00 |
| 46 | | | | | | 22 | Quinn Natalie EF Education-Cannondale | EF Education-Cannondale | | ,,0:00 |
| 47 | | | | | | 20 | Steinmetz Nicole Cynisca Cycling | Cynisca Cycling | | ,,0:00 |
| 48 | | | | | | 29 | Garza Ana Belen Boneshaker Project presented by ORANGE SEAL | Boneshaker Project presented by ORANGE SEAL | | ,,0:00 |
| 49 | | | | | | 22 | Ryan Ruby | | | 0:140:14 |
| 50 | | | | | | 36 | Canning Rachel UTC - ButcherBox Cycling p/b LOOK | UTC - ButcherBox Cycling p/b LOOK | | 0:150:15 |
| 51 | | | | | | 25 | O'Neill Cara Cynisca Cycling | Cynisca Cycling | | ,,0:15 |
| 52 | | | | | | | Reeve Olivia United Cycling | United Cycling | | 0:160:16 |
| 53 | | | | | | | Jen-La Plante Ilan | | | ,,0:16 |
| 54 | | | | | | 16 | Jaramillo Alexis Fount Cycling Guild | Fount Cycling Guild | | ,,0:16 |
| 55 | | | | | | 20 | Lawson Mairen | | | ,,0:16 |
| 56 | | | | | | 17 | Ciambotti Bridget | | | ,,0:16 |
| 57 | | | | | | 18 | Lopez Vida | | | ,,0:16 |
| 58 | | | | | | | Schugar Sofia Monarch Racing | Monarch Racing | | ,,0:16 |
| 59 | | | | | | 25 | Mauvais Chloe Monarch Racing | Monarch Racing | | ,,0:16 |
| 60 | | | | | | 23 | Hempstead Lucy | | | ,,0:16 |
| 61 | | | | | | 25 | Spearing Kyleigh | | | ,,0:16 |
| 62 | | | | | | 36 | Wrye-Simpson Brenna | | | ,,0:16 |
| 63 | | | | | | 19 | Brenneman Ella CCB p/b Levine Law Group | CCB p/b Levine Law Group | | ,,0:16 |
| 64 | | | | | | 21 | Hasse Betty CCB p/b Levine Law Group | CCB p/b Levine Law Group | | ,,0:16 |
| 65 | | | | | | 34 | Wiseman Eleanor Baloise-WB Ladies | Baloise-WB Ladies | | ,,0:16 |
| 66 | | | | | | | Henderson Amy | | | ,,0:16 |
| 67 | | | | | | 28 | Langley Emma Virginia's Blue Ridge - TWENTY24 | Virginia's Blue Ridge - TWENTY24 | | ,,0:16 |
| 68 | | | | | | 29 | Vegh Autumn | | | ,,0:16 |
| 69 | | | | | | 25 | Barrera Anet DNA Pro Cycling | DNA Pro Cycling | | ,,0:16 |
| 70 | | | | | | 27 | Tatko Rachael | | | ,,0:16 |
| 71 | | | | | | 18 | Sabo Ella Virginia's Blue Ridge - TWENTY24 | Virginia's Blue Ridge - TWENTY24 | | ,,0:16 |
| 72 | | | | | | 16 | Swierenga Sidney TaG Cycling | TaG Cycling | | ,,0:16 |
| 73 | | | | | | | Redman Paige | | | ,,0:16 |
| 74 | | | | | | 31 | Snover Ellexi Fount Cycling Guild | Fount Cycling Guild | | ,,0:16 |
| 75 | | | | | | 18 | Bond Jorja | | | ,,0:16 |
| 76 | | | | | | 20 | Lebreton Madeline | | | ,,0:16 |
| 77 | | | | | | 33 | Rinaldo Marjorie Fount Cycling Guild | Fount Cycling Guild | | ,,0:16 |
| 78 | | | | | | 22 | Edwards Tess Cynisca Cycling | Cynisca Cycling | | 0:260:26 |
| 79 | | | | | | 25 | Quiñones Laurel Virginia's Blue Ridge - TWENTY24 | Virginia's Blue Ridge - TWENTY24 | | ,,0:26 |
| 80 | | | | | | 30 | Ehrlich Emily Virginia's Blue Ridge - TWENTY24 | Virginia's Blue Ridge - TWENTY24 | | ,,0:26 |
| 81 | | | | | | 20 | Hayes Sabrina CCB p/b Levine Law Group | CCB p/b Levine Law Group | | 0:480:48 |
| 82 | | | | | | 17 | Pedersen Larissa TaG Cycling | TaG Cycling | | 4:144:14 |
| 83 | | | | | | 30 | Sheridan Katherine | | | 4:524:52 |
| 84 | | | | | | 17 | Jones Helena | | | 5:075:07 |
| 85 | | | | | | | Gammelgard Amy Monarch Racing | Monarch Racing | | ,,5:07 |
| 86 | | | | | | | Caspard Virginie United Cycling | United Cycling | | ,,5:07 |
| 87 | | | | | | 33 | Wickstrom Hayley 3T Q+M Cycling | 3T Q+M Cycling | | ,,5:07 |
| 88 | | | | | | 27 | Wu-Yan Elena EJ Dental | EJ Dental | | ,,5:07 |
| 89 | | | | | | 45 | Thorvilson Leah 3T Q+M Cycling | 3T Q+M Cycling | | ,,5:07 |
| 90 | | | | | | 23 | Tullis Jane Competitive Edge Racing | Competitive Edge Racing | | ,,5:07 |
| DNF | | | | | | | Marjorie Bemis | | | |
| DNF | | | | | | | Real Jen | | | |
| DNF | | | | | | 45 | Tracy Christie 3T Q+M Cycling | 3T Q+M Cycling | | |
| DNF | | | | | | 22 | Rich Isabella Competitive Edge Racing | Competitive Edge Racing | | |
| DNF | | | | | | 16 | Scott Veronica Competitive Edge Racing | Competitive Edge Racing | | |
| DNF | | | | | | 25 | Hachmann Ava Denver Disruptors | Denver Disruptors | | |
| DNF | | | | | | 25 | Pham Cynthia | | | |
| DNF | | | | | | 28 | Kelly Siobhan TaG Cycling | TaG Cycling | | |
| DNF | | | | | | | Lin Katherine | | | |
| DNF | | | | | | 22 | Lackman Lauren | | | |
| DNF | | | | | | | Flamm Saralita | | | |
| DNS | | | | | | 25 | Payer Kira Boneshaker Project presented by ORANGE SEAL | Boneshaker Project presented by ORANGE SEAL | | |
| DNS | | | | | | 40 | Nelson Cassandra | | | |
| DNS | | | | | | 21 | Davis Kayla | | | |