| 1 | TROLDI Giovanna | 38y + 228d | 2007 | Stage 4b | Orduña - Bilbao |
| 2 | TEUTENBERG Ina-Yoko | 37y + 223d | 2012 | Stage 1 | Iurreta - Matiena |
| 3 | LONGO Jeannie | 36y + 230d | 1995 | Stage 3 | - |
| 4 | VAN VLEUTEN Annemiek | 35y + 224d | 2018 | Stage 2 (ITT) | Agurain - Vitoria-Gasteiz |
| 5 | GARFOOT Katrin | 35y + 223d | 2017 | Stage 3 | Antzuola - Antzuola |
| 6 | SLYUSAREVA Olga | 34y + 46d | 2003 | Stage 2 | Kortezubi - Sopelana |
| 7 | ARNDT Judith | 33y + 323d | 2010 | Stage 2 | Lekeitio - Lekeitio |
| 8 | POLIKEVIČIŪTĖ Jolanta | 33y + 262d | 2004 | Stage 4 | Sopelana - Sopelana |
| 9 | KUPFERNAGEL Hanka | 33y + 89d | 2007 | Stage 4b | Orduña - Bilbao |
| 10 | ZIJLAARD-VAN MOORSEL Leontien | 33y + 84d | 2003 | Stage 3a | Sopelana - Elorrio |
| 11 | BRONZINI Giorgia | 32y + 257d | 2016 | Stage 3 | Berriatua - Berriatua |
| 12 | JOHANSSON Emma | 32y + 205d | 2016 | Stage 2 | Etxarri - Urkiola |
| 13 | STOLBOVA Svetlana | 32y + 157d | 2005 | Stage 1 | Iurreta - Elorrio |
| 14 | LUPERINI Fabiana | 32y + 147d | 2006 | Stage 3a (ITT) | Iurreta - Goiurria |
| 15 | SOMARRIBA Joane | 31y + 305d | 2004 | Stage 3b (ITT) | Elorrio - Elgeta |
| 16 | SPRATT Amanda | 31y + 248d | 2019 | Stage 2 | Aduna - Amasa |
| 17 | MOOLMAN Ashleigh | 31y + 163d | 2017 | Stage 5 | Errentería - Errentería |
| 18 | GUARNIER Megan | 30y + 349d | 2016 | Stage 4 | Portugalete - Portugalete |
| 19 | LONGHIN Katia | 30y + 190d | 2004 | Stage 1 | Iurreta - Arrasate |
| 20 | STAHURSKAYA Zinaida | 30y + 127d | 2001 | Stage 2a (ITT) | Gordexola - Gordexola |
| 21 | LJUNGSKOG Susanne | 30y + 86d | 2006 | Stage 3a (ITT) | Iurreta - Goiurria |
| 22 | BASTIANELLI Marta | 30y + 18d | 2017 | Stage 1 | Iurreta - Iurreta |
| 23 | WILES Tayler | 29y + 308d | 2019 | Stage 3 | Murgia - Santa Teodosia |
| 24 | POOLEY Emma | 29y + 249d | 2012 | Stage 2 | Lekeitio - Lekeitio |
| 25 | D'HOORE Jolien | 29y + 69d | 2019 | Stage 1 | Iurreta - Iurreta |
| 26 | CAPPELLOTTO Alessandra | 28y + 293d | 1997 | Stage 1 | - |
| 27 | PASCUAL Gema | 28y + 156d | 2007 | Stage 5 | Bilbao - Iuretta |
| 28 | MELCHERS Mirjam | 27y + 262d | 2003 | Stage 4 | Elgeta - Iurreta |
| 29 | LONGO BORGHINI Elisa | 27y + 166d | 2019 | Stage 4 | Oñati - Oñati |
| 30 | RUANO Dori | 27y + 157d | 1996 | Stage 2b | - |
| 31 | CAPPELLOTTO Valeria | 27y + 141d | 1997 | Stage 3 | - |
| 32 | VILLUMSEN Linda | 27y + 61d | 2012 | Stage 3 (ITT) | Orduna - Orduna |
| 33 | COOKE Nicole | 27y + 60d | 2010 | Stage 3 (ITT) | Elorrio - Elgeta |
| 34 | VOS Marianne | 27y + 33d | 2014 | Stage 4 | Ataun - Ataun |
| 35 | PIETERS Amy | 26y + 355d | 2018 | Stage 3 | Aretxabaleta - Aretxabaleta |
| 36 | LECOURTOIS Fany | 26y + 342d | 2000 | Stage 3 | Elgeta - Goiuria |
| 37 | HENTTALA Lotta | 26y + 290d | 2016 | Prologue | Iurreta - Iurreta |
| 38 | PUČINSKAITĖ Edita | 26y + 201d | 2002 | Stage 2b (ITT) | Elorrio - Elorrio |
| 39 | MANSVELD Debby | 26y + 177d | 1999 | Stage 1 | - |
| 40 | ZOCCA Greta | 26y + 170d | 2001 | Stage 1 | Irieta - El Lorio |
| 41 | SCHLEICHER Regina | 26y + 88d | 2000 | Stage 1 | Iurreta - Durango |
| 42 | ILAVSKÁ Lenka | 26y + 43d | 1998 | Stage 2b | - |
| 43 | VAN DEN BROEK-BLAAK Chantal | 25y + 234d | 2015 | Stage 3 | Iurreta - Iturmendi |
| 44 | BLÁZQUEZ Fátima | 25y + 35d | 2000 | Stage 2b (ITT) | Elorrio - Elgeta |
| 45 | PACZOVA Ildiko | 24y + 318d | 1993 | Stage 2b | - |
| 46 | STULTIENS Sabrina | 24y + 315d | 2018 | Stage 1 | Legazpi - Legazpi |
| 47 | BARILLOVA Elena | 23y + 285d | 1993 | Stage 2b | - |
| 48 | WORRACK Trixi | 23y + 256d | 2005 | Stage 4 | - Bilbao |
| 49 | HAYEVA Volha | 23y + 221d | 2006 | Stage 4 | Bilbao - Bilbao |
| 50 | ITURRIAGA Eneritz | 22y + 268d | 2003 | Stage 1 | Durango - Santimamiñe |