Best time trial competitors based on the PCS time trial ranking.
| Pos. | Rider | Team | Points | Time trial ranking position |
|---|---|---|---|---|
| 1 | Vargas Carolina | Eneicat - CMTeam - Seguros Deportivos | 208 | 21 |
| 2 | Hernández Lina Marcela | Colombia Pacto Por El Deporte - GW Shimano | 191 | 25 |
| 3 | Marcolini Emily | ROXO Racing | 186 | 28 |
| 4 | Peñuela Diana | DNA Pro Cycling | 176 | 32 |
| 5 | Barrera Anet | DNA Pro Cycling | 172 | 34 |
| 6 | Villalón Aranza Valentina | Eneicat - CMTeam - Seguros Deportivos | 140 | 47 |
| 7 | Guluma Sérika | La Roue Vetement Team | 129 | 53 |
| 8 | Soto Jasmin Gabriela | Liro Sport-Arraz Casanare | 120 | 59 |
| 9 | Franz Heidi | DNA Pro Cycling | 96 | 77 |
| 10 | Sánchez Maria Fernanda | Tour Y Nativa Procycling | 95 | 78 |
| 11 | Fagua Ana Milena | La Roue Vetement Team | 90 | 88 |
| 12 | Rojas Lina Mabel | Team Sistecredito | 80 | 100 |
| 13 | Moreno Sara Juliana | Colombia Pacto Por El Deporte - GW Shimano | 68 | 116 |
| 14 | Sanabria Ana Cristina | Colombia Pacto Por El Deporte - GW Shimano | 66 | 117 |
| 15 | Saavedra Anette Oriana | Team Fundecom - C. Goci | 64 | 121 |
| 16 | Alzate Andrea | Eneicat - CMTeam - Seguros Deportivos | 51 | 140 |
| 17 | Prieto Marcela Elizabeth | Pato Bike BMC Team | 50 | 143 |
| 18 | Sanchez Jennifer Camila | Fun Team Recapi-Krieger | 50 | 146 |
| 19 | Galarza Esther Jessica | Avinal - Carmen de Viboral | 47 | 152 |
| 20 | Cortes Genesis | Clarus Merquimia Group - Strongman | 46 | 153 |